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YEAH! REsults are out!
SO glad that I managed to actually inject tracer into the brain this time round.
Compared to last weeks dismal results with only a scratch made on the surface of the brain today has been a great achievement to me
Although my mentor jokingly said that it might just have been luck but still he said that sample had passed and I could use it. SO .... hip hip hurray!
For the record I injected my tracer into the endopiriform cortex which is just adjacent to my target of the piriform cortex.
but all good.... gained some confidence.
But this lao tou had to go spoil it with the beginners luck business and said that he feared that i might have gotten my central bregma landmark wrong which resulted in this off injection. Because according to the stereotaxic coordinates which are already very very lateral I should have hit the piriform cortex with the original coordinates.
so know we've calculated another +0.2mm to the lateral coordinates which is at a point that's seriously endanger of hitting completely out of the brain physically.
and he said it would be my call if i wanted to inject my three mice tomorrow using these new coordinates.
ok...unlike what u guys may be thinking of a mass production of mice in research labs, we are currently facing rather dismal numbers and as the attached student, my project basically gets funded with charity donations of mice. basically I get by each week with what mice i can get. which is truly a sad sad thing. but oh well as long as i can get as much experience as possible and can bring my project as far as it can i don't mind. OH WOE IS ME@!!!!!
yup, so basically after one hours worth of pondering and looking for articles that could confirm whether it would be a foolish venture to use those coordinates I had to swallow my pride and elation and find Ma laoshi and ask if he would be free tomorrow morning to come check if i am getting my mid points correct.
Oh woe is me.....well at least he's kind enough to volunteer his time.
haha...might attribut that to the fact that I basically threw a fit yesterday in the lab when these bloody poly students were busy yakking away in the lab and then this ma lao tou had to come and disturb me with idle chatter whilst i was busy trying to concentrate on cutting my precious samples in the cryostat, one such irritating encounter of which resulted in me ripping one sample in half which basically set off my fuse. Needless to say I kicked back my chair attempting to save the shredded piece and in the process telling the rest of the lab to not talk to me whilst i was doing that. hahaha....
Thank goodness it was the sample that wasn't injected with anything.
Wish me luck in tomorrows procedures!
Cheers!