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End of the week at last.
Been hectic hectic hectic.
Having a mentor means that u have to help him carry out his experiments and process his results. That is for the one week that u are waiting for your own mice to have survived the one week before u can perfuse them to get results.
They often do this under the pretense that helping them do all these will help improve your skills.
well yeah that's kinda true but having to mount slides is just a REAL pain in the ass.
yeah so 5 mice perfused this week, 5 brains frozen and cut, 10 wells for DAB staining...10 bloody slides to mount.
MY GOD!
yup and today i had to assist in observing 12 day old mice as they were induced into status epilepticus.
yup it's cruel but seeing those buggers stopping their epileptic dance way before the right amount of time needed to generate some form of neuronal damage to their brain is also damn frustrating.
It gets even worse when they play dead. MAN! There u are running around tryin to get someone to be there when they actually die to perfuse them. Because fine, I know how to perfuse mice now, but at the sizes that they are at at 12 days, which is basically about about half the length of your index finger, trying to poke a needle into their hearts for transpericardial perfusion is a MAJOR feat. Basically one false move and there goes everything.
Yup so whilst your running around and can't find anyone, u run back to the lab steeling urself to do the unthinkable and then u see the stupid blighter jerkily crawling around the box. This all adds up to u wanting to stimulate it even more so that it gets one hell of a massive fit.
Touche~!
right so that's basically me for this week.
next week i will perfuse the 2 mice that i injected with tracer this week and hopefully this time there will be some positive results.
PLEASE~! PLEASE GOD PLEASE~!