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beginning of a new week once again.
This week I get to process my results.
my goodness.....so unnerving.....hope i get some positive results so that I can proceed.
well lets talk about interesting things that occured this apprently boring day.
today I had to start with the first step of the protocol for processing tracer resutls.
so first agenda of the day was to perfuse my two mice, retrieve their brains and after putting them in sucrose solution wait overnight till i can proceed to cut and wash and put primary antibody on my specimen tml.
so basically i was done within the first two hours of the morning.
After lunch, fell asleep at my desk after reading the world news. yeah...i wonder why.
woke up and was steeling myself for another 4h of readin papers and stuff when ma lao tou walked past my desk and I suppose did the foul mistake of checking on my progress.
And i told him that I was bored.
And so he invited me to go down to the 2nd floor to look at slides with the confocal laser microscope.
It's really a cool device that costs about the same as my entire medical education.
complete with its own suspension table, laser machine and stuff.
The only things that u can choose to operate manually are focusing and placing the slide on the stage and the rest is controlled via a software on a computer.
so basically u can view ur brain slices with fluorescent light which allows u to view the cells labelled with tracer in green or red light.
Then using the laser, u can cut these 40 micrometre slices into even thinner slices so that u can make a 3D composite.
so cells that overlap show a positive connection between them which u determine with positive antigen antibody reaction.
yup so that was the interesting part of my day.
The next interesting thing was a matter of coincidence.
this morning I thought i was going to be late, so on reaching Novena I was running towards NNI.
Then I noticed that hurrying along side me was Dr. Tang, the lao ban and Principle investigator of our lab. He apparently didn't see me and I figured that I'm safe since i'm goin to be just as late as he is.
well so anyway we reached the lift lobby and then he saw me we said good morning and started to discuss my project. so all in all it was good. for the record I was only 5 minutes late.
Right so coincidences aside....I was headed back home this evening and guess who was
at the lift lobby on the 6th floor now?
Lao ban again.
haha....we were laughin about this complete coincidence.
Yup so all in all it was a day of coincidences and big expensive machines.
cheers!